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mek-2 rnai vector  (Source BioScience plc)

 
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    Source BioScience plc mek-2 rnai vector
    Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS-ERK-like pathway. (a) Percent of wild-type and lin-45(n2018), <t>mek-2(ku114)</t> , mpk-1(n5639) and lin-45(n2018); gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of >100 animals (b) Percent of wild-type and lin-45(n2018) cross progeny failing to arrest development at 500 mM NaCl after 48 hrs. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. (c) Representative germlines dissected from wild-type animals exposed to either 50 mM NaCl or 300 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red) Each condition was replicated 16 times. Scale bar 50 μm (d) Relative expression of gpdh-2 mRNA in wild-type and lin-45(n2018) embryos measured by qRT-PCR and normalized to the expression of the histone his-24. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos (e) Glycerol-to-glucose ratio in wild-type and lin-45(n2018) mutant embryos. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos. The quantified results are presented as mean ± s.d. using ANOVA (a, b) and two-tailed t-test (d, e). * P < 0.05, ** P < 0.01, *** P < 0.001 were considered significant. n.s., not significant. See Statistics Source Data in
    Mek 2 Rnai Vector, supplied by Source BioScience plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mek-2 rnai vector/product/Source BioScience plc
    Average 90 stars, based on 1 article reviews
    mek-2 rnai vector - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Insulin-like signalling to the maternal germline controls progeny response to osmotic stress"

    Article Title: Insulin-like signalling to the maternal germline controls progeny response to osmotic stress

    Journal: Nature cell biology

    doi: 10.1038/ncb3470

    Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS-ERK-like pathway. (a) Percent of wild-type and lin-45(n2018), mek-2(ku114) , mpk-1(n5639) and lin-45(n2018); gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of >100 animals (b) Percent of wild-type and lin-45(n2018) cross progeny failing to arrest development at 500 mM NaCl after 48 hrs. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. (c) Representative germlines dissected from wild-type animals exposed to either 50 mM NaCl or 300 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red) Each condition was replicated 16 times. Scale bar 50 μm (d) Relative expression of gpdh-2 mRNA in wild-type and lin-45(n2018) embryos measured by qRT-PCR and normalized to the expression of the histone his-24. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos (e) Glycerol-to-glucose ratio in wild-type and lin-45(n2018) mutant embryos. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos. The quantified results are presented as mean ± s.d. using ANOVA (a, b) and two-tailed t-test (d, e). * P < 0.05, ** P < 0.01, *** P < 0.001 were considered significant. n.s., not significant. See Statistics Source Data in
    Figure Legend Snippet: Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS-ERK-like pathway. (a) Percent of wild-type and lin-45(n2018), mek-2(ku114) , mpk-1(n5639) and lin-45(n2018); gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of >100 animals (b) Percent of wild-type and lin-45(n2018) cross progeny failing to arrest development at 500 mM NaCl after 48 hrs. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. (c) Representative germlines dissected from wild-type animals exposed to either 50 mM NaCl or 300 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red) Each condition was replicated 16 times. Scale bar 50 μm (d) Relative expression of gpdh-2 mRNA in wild-type and lin-45(n2018) embryos measured by qRT-PCR and normalized to the expression of the histone his-24. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos (e) Glycerol-to-glucose ratio in wild-type and lin-45(n2018) mutant embryos. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos. The quantified results are presented as mean ± s.d. using ANOVA (a, b) and two-tailed t-test (d, e). * P < 0.05, ** P < 0.01, *** P < 0.001 were considered significant. n.s., not significant. See Statistics Source Data in

    Techniques Used: Staining, Expressing, Quantitative RT-PCR, Mutagenesis, Two Tailed Test



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    mek-2 rnai vector  (Source BioScience plc)
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    Source BioScience plc mek-2 rnai vector
    Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS-ERK-like pathway. (a) Percent of wild-type and lin-45(n2018), <t>mek-2(ku114)</t> , mpk-1(n5639) and lin-45(n2018); gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of >100 animals (b) Percent of wild-type and lin-45(n2018) cross progeny failing to arrest development at 500 mM NaCl after 48 hrs. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. (c) Representative germlines dissected from wild-type animals exposed to either 50 mM NaCl or 300 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red) Each condition was replicated 16 times. Scale bar 50 μm (d) Relative expression of gpdh-2 mRNA in wild-type and lin-45(n2018) embryos measured by qRT-PCR and normalized to the expression of the histone his-24. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos (e) Glycerol-to-glucose ratio in wild-type and lin-45(n2018) mutant embryos. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos. The quantified results are presented as mean ± s.d. using ANOVA (a, b) and two-tailed t-test (d, e). * P < 0.05, ** P < 0.01, *** P < 0.001 were considered significant. n.s., not significant. See Statistics Source Data in
    Mek 2 Rnai Vector, supplied by Source BioScience plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mek-2 rnai vector/product/Source BioScience plc
    Average 90 stars, based on 1 article reviews
    mek-2 rnai vector - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

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    Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS-ERK-like pathway. (a) Percent of wild-type and lin-45(n2018), mek-2(ku114) , mpk-1(n5639) and lin-45(n2018); gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of >100 animals (b) Percent of wild-type and lin-45(n2018) cross progeny failing to arrest development at 500 mM NaCl after 48 hrs. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. (c) Representative germlines dissected from wild-type animals exposed to either 50 mM NaCl or 300 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red) Each condition was replicated 16 times. Scale bar 50 μm (d) Relative expression of gpdh-2 mRNA in wild-type and lin-45(n2018) embryos measured by qRT-PCR and normalized to the expression of the histone his-24. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos (e) Glycerol-to-glucose ratio in wild-type and lin-45(n2018) mutant embryos. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos. The quantified results are presented as mean ± s.d. using ANOVA (a, b) and two-tailed t-test (d, e). * P < 0.05, ** P < 0.01, *** P < 0.001 were considered significant. n.s., not significant. See Statistics Source Data in

    Journal: Nature cell biology

    Article Title: Insulin-like signalling to the maternal germline controls progeny response to osmotic stress

    doi: 10.1038/ncb3470

    Figure Lengend Snippet: Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS-ERK-like pathway. (a) Percent of wild-type and lin-45(n2018), mek-2(ku114) , mpk-1(n5639) and lin-45(n2018); gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of >100 animals (b) Percent of wild-type and lin-45(n2018) cross progeny failing to arrest development at 500 mM NaCl after 48 hrs. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. (c) Representative germlines dissected from wild-type animals exposed to either 50 mM NaCl or 300 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red) Each condition was replicated 16 times. Scale bar 50 μm (d) Relative expression of gpdh-2 mRNA in wild-type and lin-45(n2018) embryos measured by qRT-PCR and normalized to the expression of the histone his-24. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos (e) Glycerol-to-glucose ratio in wild-type and lin-45(n2018) mutant embryos. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos. The quantified results are presented as mean ± s.d. using ANOVA (a, b) and two-tailed t-test (d, e). * P < 0.05, ** P < 0.01, *** P < 0.001 were considered significant. n.s., not significant. See Statistics Source Data in

    Article Snippet: Wild-type embryos fed Escherichia coli HT115 containing either the empty vector L4440 or a mek-2 RNAi vector (Ahringer library - Source Biosciences) were grown at 20°C for 72 hrs.

    Techniques: Staining, Expressing, Quantitative RT-PCR, Mutagenesis, Two Tailed Test